返回
返回
返回
Red blood cell lysis buffer
One click copying of product information
-
AP01L106100mL78
-
AP01L107500 mL298
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
-
Product Description
This product exploits the principle that osmotic pressure differences across the cell membrane can cause it to lyse, thereby lysing red blood cells. It is primarily used in experiments such as the isolation and purification of enzymatically digested tissue cells, the isolation and purification of lymphocytes, and the removal of red blood cells during the extraction of proteins and nucleic acids from tissue cells.
Order Information
Product Name
Item number
Specifications
Red blood cell lysis buffer
AP01L106
100 mL
Red blood cell lysis buffer
AP01L107
500 mL
Transportation and Storage
Store at room temperature. Keep refrigerated at 4°C; shelf life is 12 months.
Instructions for Use
1. Lysis of red blood cells
(1) Add three times the volume of red cell lysis buffer to one sample of fresh whole blood to be lysed (e.g., add 3 mL of red cell lysis buffer to 1 mL of fresh whole blood), and gently vortex or invert to mix thoroughly. [Note]: If processing fresh tissue cells, first digest the tissue with trypsin or a similar enzyme to obtain a single-cell suspension, then centrifuge to pellet the cells before proceeding with subsequent steps.
(2) Incubate on ice for 15 min, gently vortexing to mix twice during this period; [Note]: After red blood cell lysis, the solution should be clear and transparent.
2. Collect white blood cells
(1) At 4°C, centrifuge at 450×g for 10 min to pellet the leukocytes, then carefully aspirate and discard the supernatant.
(2) Add twice the volume of red blood cell lysis buffer to the above pellet, and gently vortex to fully resuspend the leukocytes (e.g., if the initial blood volume is 1 mL, add 2 mL of red blood cell lysis buffer).
(3) At 4°C, centrifuge at 450×g for 10 min to pellet the leukocytes, then carefully and completely aspirate the supernatant.
(4) Resuspend the cells for subsequent experiments. [Note]: If RNA is to be extracted, it is best to use RNase-free solutions starting from this step.
Precautions
1. This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
2. For your own safety, please take appropriate protective measures before using reagents, such as wearing a lab coat and gloves.
Related Product Recommendations
EzTissue Plus Tissue Transport and Preservation Solution (Catalog No.: AC05L362)
Collagenase, Type I (Catalog No.: AC15L041)
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
-
Fill in the product batch number for inquiry
-
Fill in the product batch number for inquiry
Best Sellers
Popular Articles
Group Website
-
Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
WeChat Official Account
Online Customer Service 1
Online Customer Service 2
Telephone consultation
