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LiFluor 488 succinimidyl ester
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AP35L1541 mg3280
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AP35L15510 mg22800
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Product Description
LiFluor 488 succinimidyl ester is one of the LiFluor series of fluorescent labeling dyes, capable of covering the entire visible spectrum. All LiFluor dyes exhibit excellent water solubility, and their hydrophilic nature minimizes the need for organic solvents. Compared with conventional dyes such as FITC, TRITC, Texas Red, Cy3, Cy5, and Cy7, LiFluor dyes offer superior labeling performance. Certain LiFluor dyes outperform Alexa Fluor–labeled dyes on specific antibodies. They are highly cost-effective fluorescent dyes for labeling proteins and nucleic acids, serving as an alternative to Alexa Fluor dyes. Each LiFluor dye has been specifically developed to match the spectral characteristics of a particular Alexa Fluor or other labeling dye, such as DyLight dyes.
N‑succinimidyl esters have been shown to be effective reagents for amine modification, as the resulting amide bonds are essentially identical to native peptide linkages and are highly stable. These reagents are generally stable and exhibit good reactivity and selectivity toward aliphatic amines. When using succinimidyl ester compounds in conjugation reactions, only a few factors need to be considered: 1. Solvent: In most cases, reactive dyes should be dissolved in anhydrous dimethylformamide (DMF) or dimethyl sulfoxide (DMSO). 2. Reaction pH: The labeling reaction between amines and succinimidyl esters is strongly pH‑dependent. Aminoreactive reagents react with deprotonated aliphatic amine groups, including terminal amines on proteins and the β‑amino group of lysine residues. Consequently, amidation reactions are typically carried out at pH values above 7.5; proteins modified with succinimidyl esters are representative examples, usually synthesized at pH 8.5–9.5. 3. Reaction buffer: When employing aminoreactive reagents, buffers containing free amines—such as Tris and glycine—as well as thiol‑containing compounds must be avoided. Ammonium salts commonly used for protein precipitation, such as ammonium sulfate and ammonium acetate, should also be removed prior to dye conjugation, for example by dialysis. 4. Reaction temperature: Most conjugation reactions proceed at room temperature. Certain labeling reactions may require elevated or reduced temperatures. LiFluor series dyes serve as alternatives to AF‑series dyes.
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Product Name
Item number
Specifications
LiFluor 488 succinimidyl ester
AP35L154
1 mg
LiFluor 488 succinimidyl ester
AP35L155
10 mg
Transportation and Storage
Blue ice transport. Store at -20°C away from light; shelf life: 12 months.
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This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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