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Eosin staining solution
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AS11L031100mL140
- Product Description
- Product FAQ
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- Product Literature
- MSDS
- Operation video
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Product Description
Eosin is the most commonly used cytoplasmic stain. It was first employed by List in 1885. When used in combination with basic blue dyes and paired with hematoxylin, it forms the H&E stain. Eosin is an acidic dye that interacts with proteins in the cell rich in basic amino acids. The resulting dye–protein complexes are characterized by a pinkish staining of the cytoplasm.
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Specifications
Eosin staining solution
AS11L031
100mL
Transportation and Storage
Store at room temperature, protected from light. Shelf life: 12 months.
Instructions for Use
1. Deparaffinization and fixation of frozen sections:
2. Hematoxylin staining;
3. Rinse with tap water;
4. If a regression‑type hematoxylin stain is used, destaining is required, followed by rinsing with tap water.
5. Blueing using an alkaline solution or Scott's Tap Water Substitute:
6. Rinsing with tap water:
7. Rinse the slides in 95% ethanol for 30 seconds;
8. Counterstain with eosin solution (alcohol-soluble) for 30 seconds to 3 minutes;
9. Dehydrate, clear, and mount the slide.
Staining results: The cytoplasm is stained pink to red, while the nucleus is stained blue to dark blue, depending on the hematoxylin solution used.
Precautions
1. This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
2. For your safety and health, please wear a lab coat and disposable gloves when performing this procedure.
3. This product is alcohol-soluble, with a concentration of 0.1%, and can be used in conjunction with hematoxylin staining solution (Catalog No.: AS11L021).
4. When using this product for the first time, it is recommended to prepare 1–2 samples for a preliminary experiment.
Related Product Recommendations
General-purpose tissue fixation solution (4% paraformaldehyde) (Catalog No.: AC28L112)
Antifade reagent (Catalog No.: AC28L512)
Hematoxylin and Eosin (HE) Staining Kit (Catalog No.: AS11L011)
Hematoxylin Staining Solution (Catalog No.: AS11L021)
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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