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Ready-to-use BCA protein quantitation kit
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AP12L135500T268
- Product Description
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Product Description
The BCA (bicinchoninic acid) assay is currently one of the most widely used methods for determining protein concentration. It is based on the biuret reaction: under alkaline conditions, proteins reduce Cu²⁺ to Cu⁺, which then forms a purple-colored complex with the BCA reagent, exhibiting a strong absorbance at 562 nm. The amount of this reaction product is directly proportional to the protein concentration. By measuring the absorbance at 562 nm and comparing it to a standard curve, the concentration of the protein sample can be calculated. This method is rapid, sensitive, stable, and reliable, with a very low coefficient of variation across different protein types. The kit includes a stable protein standard solution for constructing the standard curve.
The BCA protein assay kit can be used for both tube‑based and microplate‑based assays. The tube method requires a relatively large protein sample (100 μL) and working reagent (2 mL), with a protein-to‑working‑reagent ratio of 1:20 (v/v). The measurable protein concentration range is 20–2000 μg/mL; with the enhanced protocol, detection down to 5 μg/mL is possible. The microplate method is simple and convenient, requiring only a small volume of protein sample (10–25 μL) and working reagent (200 μL), at a protein-to‑working‑reagent ratio of either 1:20 (v/v) or 1:8 (v/v). The measurable protein concentration range is 20–2000 μg/mL, and the enhanced protocol allows detection down to 5 μg/mL.
Our company offers a ready-to-use BCA protein assay kit, complete with pre‑prepared standards, eliminating the need for time‑consuming dilution steps. The cuvette‑based method enables 50 assays, while the microplate‑based method supports up to 500 assays.
Order Information
Product Name
Item number
Specifications
Ready-to-use BCA protein quantitation kit
AP12L135
500T
Product ingredients
Component
Specifications
Final BSA concentration
BCA Reagent A
100 mL
○
BCA Reagent B
5 mL
○
BSA standard solution①
0.5 mL
2000 μg/mL
BSA standard solution②
0.5 mL
1500 μg/mL
BSA standard solution③
0.5 mL
1000 μg/mL
BSA standard solution④
0.5 mL
750 μg/mL
BSA standard solution⑤
0.5 mL
500 μg/mL
BSA standard solution⑥
0.5 mL
250 μg/mL
BSA standard solution⑦
0.5 mL
125 μg/mL
BSA standard solution⑧
0.5 mL
25 μg/mL
BSA standard solution⑨
0.5 mL
0 μg/mL = Blank
Transportation and Storage
Store at room temperature. Keep refrigerated at 4°C; shelf life is 12 months.
BCA Assay Interferent Compatibility Table
Name of interfering substance
Tolerable concentration
Name of interfering substance
Tolerable concentration
Sodium bica rbonate
100 mM
BugBuster Protein Extraction Reagent ( Cat. No. 70584)
n interference
( undiluted )
Sodium phos phate
25 m M
Calcium chloride ( in TBS, pH8.0 )
10 mM
2-Me rcaptoethanol
0.01%
Cell Tytic B Reagent
nointerference
( undiluted )
G glycerol ( pure )
10%
Cesium m bicarbonate
100 mM
Glycine - HCl , pH 2.8
100 mM
CHAPS
5%
HEP ES
100 mM
Cobalt chloride ( in TBS, pH8.0 )
0.8 mM
Hydrochloric acid
100 mM
CytoBuster Protein Extraction Reagent ( Cat. No . 71009)
n interference
( undiluted )
Leupeptin
10 mg/L
Deoxycholic acid
5%
Nickel chloride (
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Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
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