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2 x Taq PCR Plus Mix
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AN11L8282*1 mL45
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AN11L8295*1 mL95
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Product Description
2 x Taq PCR Plus Mix is a pre-mixed PCR reaction solution that contains Taq DNA polymerase, dNTPs, reaction buffer, loading dye, and other components. When using, simply take an appropriate volume of this product, add the template and primers, and then top up with ddH2O to bring the final reaction volume to 1×; the PCR reaction can then be performed. The PCR products have an A‑overhang at the 3′ end, allowing them to be directly used for T/A cloning after purification.
This product employs a composite enzyme system in which Taq DNA polymerase and a 3′→5′ exonuclease‑active protein work in concert, enabling high‑yield amplification under an optimized reaction buffer. It can amplify DNA fragments up to 7 kb in length and is compatible with templates containing 30%–70% GC content. Compared with conventional Taq enzyme (WT‑Taq), its extension rate is 2–4 times faster, significantly shortening the reaction time.
This product contains two dyes; after the PCR reaction is complete, no additional loading buffer is required, and electrophoresis can be performed directly. During electrophoresis, two indicator bands—blue and red—will appear. The dye does not affect PCR amplification efficiency; however, for experiments that require optical analyses such as absorbance or fluorescence measurements of the PCR products, it is recommended to purify the PCR products prior to analysis or to use a dye‑free PCR master mix.
Order Information
Product Name
Item number
Specifications
2 x Taq PCR Plus Mix
AN11L828
2*1 mL
2 x Taq PCR Plus Mix
AN11L829
5*1 mL
Transportation and Storage
Blue ice transport. Store at −20°C; shelf life: 24 months.
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This product is intended for scientific research purposes only. For research use only; not for clinical diagnosis, treatment, or other such applications.
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