返回
返回
返回
Single-component TMB chromogenic solution
One click copying of product information
-
AP37L105100mL180
-
AP37L106500 mL780
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
-
Product Description
The single‑component TMB chromogenic solution employs the latest one‑step TMB colorimetric technology, utilizing horseradish peroxidase (HRP) to catalyze TMB development and delivering low background and high sensitivity for assays such as ELISA. TMB (3,3′,5,5′‑tetramethylbenzidine) is a widely used substrate for HRP; under the catalysis of HRP or other suitable peroxidases, TMB yields a soluble blue product, with absorbance typically measurable at 370 nm or in the 620–650 nm range. Following the color development reaction, the reaction can be terminated by adding either a TMB stop solution at 450 nm or a TMB stop solution at 650 nm. After treatment with the 450 nm stop solution or sulfuric acid, the solution turns yellow and its absorbance can be measured at 450 nm; when using the 650 nm stop solution, the solution remains blue, allowing absorbance to be measured in the 620–650 nm range.
This color-developing solution is a ready-to-use, single-component formulation that requires no additional reagents such as hydrogen peroxide. It addresses the common issues of traditional multi‑component TMB substrates—namely, precipitation and unstable assay results—while simplifying the procedure and ensuring consistent, reliable outcomes. It is suitable for assays with either 37°C incubation or room‑temperature development for 15–30 minutes.
Order Information
Product Name
Item number
Specifications
Single-component TMB chromogenic solution
AP37L105
100 mL
Single-component TMB chromogenic solution
AP37L106
500 mL
Transportation and Storage
Blue ice transport. Store at 4°C; shelf life: 48 months.
Related Product Recommendations
BCA Protein Quantitation Kit (Catalog No.: AP12L025)
Three-color pre-stained protein marker (10–180 kDa) (Catalog No.: AP13L052)
EZ ECL pico Chemiluminescent Solution (Ultra-Sensitive) (Catalog No.: AP34L024)This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
-
Fill in the product batch number for inquiry
-
Fill in the product batch number for inquiry
Best Sellers
Popular Articles
Group Website
-
Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
WeChat Official Account
Online Customer Service 1
Online Customer Service 2
Telephone consultation
