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MEM (contains NEAA, does not contain HEPES)
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AC01L324500 mL55
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Product Description
MEM (Minimum Essential Medium) It is a commonly used cell culture medium. MEM can be employed for the cultivation of various suspension- and adherent‑dependent mammalian cells, including HeLa, BHK‑21, 293, HEP‑2, HT‑1080, MCF‑7, fibroblasts, and primary rat astrocytes, among others.
MEM It was developed by Harry Eagle, building on his earlier Basal Medium Eagle (BME) formulation. Since then, numerous modifications have been introduced to MEM, including Glasgow’s MEM, MEM α, DMEM, and Temin’s modified medium. MEM contains Earle’s balanced salts for CO2 incubators or Hanks’ balanced salts for CO2‑free incubators; this product is formulated with Earle’s balanced salts. MEM does not contain proteins, lipids, or growth factors; therefore, it requires supplementation, typically with 10% fetal bovine serum (FBS). MEM employs a sodium bicarbonate buffering system (2.2 g/L), necessitating an environment with 5–10% CO2 to maintain physiological pH.
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Product Name
Item number
Specifications
MEM( Contains NEAA, does not contain HEPES)
AC01L324
500mL
Transportation and Storage
Store at room temperature. Keep refrigerated at 4°C, protected from light; shelf life is 12 months. [Note]: Avoid repeated freeze–thaw cycles.
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This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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