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RNA extraction auxiliary reagent (chloroform substitute)
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AN51L67520mL58
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AN51L677100mL158
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
A chloroform substitute is a mixture of several low-toxicity organic compounds that can replace chloroform in RNA/DNA extraction, enabling phase separation, removal of pigments and proteins, and elimination of phenol. The main components of this product are 1-bromo-3-chloropropane and other additives.
Order InformationProduct Name
Item number
Specifications
RNA extraction auxiliary reagent (chloroform substitute)
AN51L675
20mL
RNA extraction auxiliary reagent (chloroform substitute)
AN51L677
100mL
Transportation and Storage
Store at room temperature, protected from light. Shelf life: 24 months.
Instructions for Use
One-step RNA extraction reagent: Add 100 µL of a chloroform substitute (to replace 200 µL of chloroform) to 1 mL of total RNA extraction reagent (Trizol; catalog number: AN51L758) or another similar extraction reagent.
CTAB extraction reagent: Add a chloroform substitute in an equal volume to the CTAB lysis buffer (chloroform is also used at an equal volume).
Phenol extraction reagent: Perform an extraction using a chloroform substitute equivalent in volume to the phenol‑saturated water or Tris‑saturated phenol, followed by a second extraction with an equal volume of the same chloroform substitute to remove residual phenol (phenol is slightly soluble in water).
Precautions
1. This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other such purposes.
2. Skin contact: Immediately remove contaminated clothing, rinse thoroughly with plenty of running water, and seek medical attention.
3. Eye contact: Immediately lift the eyelids and flush thoroughly with copious amounts of running water or saline solution for at least 15 minutes; seek medical attention.
4. Inhalation: Quickly remove the person from the exposure site to fresh air and ensure the airway remains clear. If breathing is difficult, administer oxygen; if breathing has stopped, immediately perform artificial respiration and seek medical attention.
5. Ingestion: Rinse mouth with water, have the person drink milk or egg whites, and seek medical attention.
Related Product Recommendations
Total RNA extraction reagent (Trizol) (Catalog No.: AN51L758)
Total RNA Column-Based Extraction Kit (Catalog No.: AN51L517)
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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