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NLS-Cas9 Nuclease
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AM01L1018 nmol (130 μL)9800
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
Cas9 Protein can bind to the guide. RNA ( gRNA ) forming a highly stable ribonucleoprotein ( RNP ) complex, as CRISPR/Cas9 A component of the system, Cas9 RNP The complex can, upon entering the cell, through N Nuclear localization signal (NLS), Enhance nuclear import efficiency. Li Ji Bio has meticulously screened and optimized nuclear localization signals ( NLS ), and insert multiple NLS, Provided NLS-Cas9 Nuclease Can help more efficiently RNP Enter the nucleus, increase the genome. DNA The cleavage rate exhibits consistently high editing efficiency across all validated cell lines, including standard, immune, primary, and stem cells. This product is packaged for industrial customers.
Order Information
Product Name
Item number
Specifications
NLS-Cas9 Nuclease
AM01L101
8 nmol (130 μL)
Product Specifications
Source
Reorganization Cas9 Derived from Escherichia coli
Species
Streptococcus pyogenes
Tag
None
Molecular weight
164kDa
Reaction temperature
37 °C
Solvent
25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerin, pH8.0 at 25 °C
Transportation and Storage
Dry ice transportation. -20 Store at ℃; shelf life: 12 Months.
Instructions for Use
Recommended In Vitro Cleavage Protocol
100 μL Reaction-electricity conversion system
Nuclease-free water
69 μL
10 X Cas9 Nuclease Reaction Buffer
10 μL
300 nM sgRNA
10 μL (30 nM final)
NLS-Cas9 Nuclease
1-2 μL (0.5-1 μM final)
Reaction volume
90 μL
Pre-incubate for 10 minutes at 25℃
30 nM substrate DNA
10 μL (3 nM final)
Total reaction volume
100 μL
Incubate at 37 ℃ for 1 hour and proceed with fragment analysis
Precautions
1. This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other such purposes.
2. The reference reaction system is 100 μL and can be scaled up or down proportionally.
3. During the experimental procedure, wear a mask and use nuclease-free consumables and reagents to prevent sgRNA degradation.
4. To maintain the optimal performance of this product, please do not subject it to freeze–thaw cycles.
5. 10X Reaction Buffer Composition: 200 mM HEPES, 1 M NaCl, 50 mM MgCl₂, 1 mM EDTA, pH 6.5 at 25°C 。
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This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
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