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EZ Trans Lipo Cell Transfection Reagent (Lipid-Based)
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AC04L0711 mL1300
- Product Description
- Product FAQ
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- Product Literature
- MSDS
- Operation video
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Product Description
EZ Trans Lipo Cell Transfection Reagent (Liposome) (hereinafter referred to as “EZ Trans Lipo”) is the latest cell transfection reagent developed by Li Ji Biotechnology. EZ Trans Lipo encapsulates nucleic acids within nanoscale liposomes and employs a specialized delivery mechanism to introduce exogenous DNA into cells. It demonstrates excellent transfection efficiency across a wide range of common cell lines as well as challenging-to-transfect lines, such as dendritic cells, A549, HepG2, and NIH3T3, while exhibiting low cytotoxicity and high efficiency with minimal impact on cell viability.
Following comparative testing across nearly one hundred cell types, EZ Trans Lipo demonstrated transfection efficiency and cytotoxicity profiles that were either comparable to or superior to those of imported products in the vast majority of tested cell lines. It offers advantages such as high transfection efficiency, low cytotoxicity, broad cellular applicability, and excellent cost-effectiveness.
More case studies are available on the official website under Resources & Support > Case Studies.
Product ingredients
Product Name
Specifications
EZ Trans Lipo Cell Transfection Reagent (Lipid-Based)
1 mL
Transportation and Storage
Blue ice transport. Store at 4°C; shelf life: 12 months.
Appendix: Comparison of Several Cell Transfection Methods
Transfection method
Principle
Main applications
Features
Cationic polymer
( EZ Trans )Positively charged polymers form positively charged complexes with the negatively charged phosphate groups of nucleic acids, which then interact with the negatively charged proteoglycans on the cell surface and enter the cell via endocytosis.
Stable transfection
Transient transfection
All cells
In addition to the high transfection efficiency, simple operation, broad applicability, and excellent reproducibility characteristic of cationic liposomes, it also exhibits high in vivo transfection efficiency and low cytotoxicity, making it a next-generation transfection reagent.
Cationic liposome method
(EZ Trans lipo pro)
Positively charged liposomes form complexes with the negatively charged phosphate groups of nucleic acids, after which the residual electrostatic charges on the liposomes interact with the negatively charged sialic acid residues on the cell membrane. Additionally, cells can internalize DNA‑encapsulated nanoliposomal particles via endocytosis.
Stable transfection
Transient transfection
All cells
It is easy to use, can deliver large DNA fragments, and is compatible with various types of naked DNA, RNA, or siRNA. It efficiently transfects a wide range of cell types and exhibits no immunogenicity, demonstrating high efficiency in in vitro gene delivery.
DEAE-dextran method
The complex formed by the interaction between positively charged DEAE-dextran and the negatively charged phosphate backbone of nucleic acids is internalized by cells.
Transient transfection
It is relatively simple and more reproducible than calcium phosphate, but it exhibits some cytotoxicity; serum must be removed during transfection, and it is generally used only for BSC‑1, CV‑1, and COS cell lines.
Calcium phosphate method
The calcium phosphate–DNA complex adsorbs onto the cell membrane and is internalized by the cell.
Stable transfection
Transient transfection
Not suitable for primary cells (which require a higher DNA concentration); the procedure is simple but has poor reproducibility, and some cell types are not amenable. For these cells, we recommend using CsCl gradient centrifugation; transfection results in a higher copy number.
Retrovirus (RNA)
It enters the host cell through the interaction between the viral envelope glycoprotein and the receptor on the host cell surface, after which reverse transcriptase initiates DNA synthesis and randomly integrates into the host genome.
Stable transfection
Specific host cell
It can be used for cells that are difficult to transfect, primary cells, and in vivo cells, but the gene carried must not be too large.
adenovirus
(double-stranded DNA)
It first binds to receptors on the cell surface and is then internalized by the cell via αv integrin–mediated endocytosis.
Transient transfection
Specific host cell
It can be used for cells that are difficult to transfect, but safety considerations must be taken into account.
Biolistic particle delivery method
(Gene gun particle bombardment method)
DNA is precipitated using microscopic heavy-metal particles, and the coated particles are then delivered into cells via a ballistic delivery system. Once inside the cell, the DNA is gradually released and expressed.
Transient transfection
Stable transfection
Applicable to: human epidermal cells, fibroblasts, lymphocyte lines, and primary cells.
Microinjection method
Directly inject DNA into the target cell nucleus using micromanipulation.
Stable transfection
Transient transfection
The number of transfected cells is limited, and this approach is often used for engineering or transgenic animal embryonic cells.
Electroporation method
High pulsed voltage disrupts the cell membrane potential, allowing DNA to be delivered through nanopores formed in the membrane.
Stable transfection
Transient transfection
All cells
It has broad applicability; in addition to plasmids, it can also transfect large genomes (>65 kb), but it results in high cell mortality, requires large amounts of DNA and cells, and necessitates optimization of electroporation conditions for different cell types, yielding a low copy number of 1–20.
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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A: All times are counted from the moment the EZ Trans-DNA complex comes into contact with the cells.
Q: Low transfection efficiency and high cytotoxicity—cell density has not been optimized.
Q: How do you determine the transfection efficiency in an experiment?
Q: Low transfection efficiency—centrifuge the transfection complex at high speed.
Q: High cytotoxicity — DNA amount is too high
A: It is recommended to plot a dose–response curve to determine the optimal DNA concentration.
Q: High cytotoxicity—excessive transfection reagent dosage
Q: High cytotoxicity—cell condition is poor during transfection.
Q: High cytotoxicity—antibiotics are added too early during stable transfection.
A: Please add the selection antibiotic 48 hours after transfection.
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Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
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