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EZ Trans siRNA Transfection Reagent
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AC04L051300 µL700
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AC04L0521 mL1800
- Product Description
- Product FAQ
- COA
- Product Literature
- MSDS
- Operation video
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Product Description
EZ Trans siRNA transfection reagent is a novel transfection agent developed based on cationic polymeric polymers, suitable for transfecting siRNA and microRNA (miRNA). Following an upgraded formulation, this product delivers high‑efficiency transfection with low cytotoxicity across a wide range of commonly used cell types. It also offers ease of use, excellent stability, and broad compatibility; serum and antibiotics do not interfere with transfection, and in most cell lines, media changes are unnecessary after transfection.
Order Information
Product Name
Item number
Specifications
EZ Trans siRNA Transfection Reagent
AC04L051
300 uL
EZ Trans siRNA Transfection Reagent
AC04L052
1 mL
Product ingredients
Component
AC04L051
AC04L052
A. siRNA transfection reagent
300 uL
1 mL
B. Booster
1 mL
3×1 mL
Transportation and Storage
Blue ice transport. Store at 4°C; shelf life is 12 months. If not used for an extended period, we recommend storing at −20°C. Note: Avoid repeated freeze-thaw cycles.
Instructions for Use (Taking a 24-well plate as an example; for transfection in other culture dishes, please refer to Table 1.)
1. Cell seeding
For adherent cells: The day before transfection, seed the cells at 1–2 × 10 per well. 5 Plate the cells at a density that yields 60%–80% confluence at the time of transfection.
2. Prepare the EZ Trans–siRNA complex:
(1) For each well, pipette 1 μL of siRNA solution (20 μM) and 5 μL of Booster solution, gently pipette up and down to mix thoroughly, then add 2 μL of EZ Trans siRNA transfection reagent, mix again by pipetting, and incubate at room temperature for 5 minutes. Note: This transfection reagent must be brought to room temperature and vortexed briefly before use.
(2) Add 12 μL of sterile PBS, Opti-MEM, or complete cell culture medium to the above mixture to prepare a 20 μL EZ Trans-siRNA complex.
3. Cell Transfection:
(1) Add 20 μL of the aforementioned EZ Trans-siRNA complex to one well of a 24-well plate, and gently shake the plate to mix the contents.
(2) Incubate in a 37°C, 5% CO₂ incubator for 24–48 hours, after which the gene‑knockdown efficiency can be assessed or subsequent functional assays performed.
Transfection procedure
Related Product Recommendations
CCK-8 Kit (Cell Counting Kit-8) (Catalog No.: AC11L054)
EZ Trans Cell Transfection Reagent (High Efficiency) (Catalog No.: AC04L092)
This product is intended solely for scientific research and must not be used for clinical diagnosis, treatment, or any other purposes.
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A: All times are counted from the moment the EZ Trans-DNA complex comes into contact with the cells.
Q: Low transfection efficiency and high cytotoxicity—cell density has not been optimized.
Q: How do you determine the transfection efficiency in an experiment?
Q: Low transfection efficiency—centrifuge the transfection complex at high speed.
Q: High cytotoxicity — DNA amount is too high
A: It is recommended to plot a dose–response curve to determine the optimal DNA concentration.
Q: High cytotoxicity—excessive transfection reagent dosage
Q: High cytotoxicity—cell condition is poor during transfection.
Q: High cytotoxicity—antibiotics are added too early during stable transfection.
A: Please add the selection antibiotic 48 hours after transfection.
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Contact Information
Landline phone:021-50778506
Business Manager:18616108315Business Cooperation/Scholarships:18616108697
Address:4th Floor, Building 27, Lane 908, Ziping Road, International Medical Park, Pudong New Area, Shanghai
Email:ztt0005@obiosh.com
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